Pelargonidin chloride

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

天竺葵素氯化物对 HepG2 细胞中 CTN 诱导的氧化应激具有保护作用，并通过 Keap1/Nrf2 信号通路上调解毒酶的活性水平。PC 在改善柑橘素 (CTN) 诱导的细胞毒性和氧化应激中的作用。

Pelargonidin chloride shows protective effect against CTN-induced oxidative stress in HepG2 cells and up-regulated the activity of detoxification enzyme levels through Keap1/Nrf2 signaling pathway.The effect of PC in ameliorating citrinin (CTN) induced cytotoxicity and oxidative stress. The cytotoxicity of CTN was evaluated by treating HepG2 (Human hepatocellular carcinoma) cells with CTN (0-150 μM) in a dose dependent manner for 24 h, and the IC50 was determined to be 96.16 μM. CTN increased lactate dehydrogenase leakage (59%), elevated reactive oxygen species (2.5-fold), depolarized mitochondrial membrane potential as confirmed by JC-1 monomers and arrested cell cycle at G2/M phase. Further, apoptotic and necrotic analysis revealed significant changes followed by DNA damage. To overcome these toxicological effects, PC was pretreated for 2 h followed by CTN exposure for 24 h. Pretreatment with PC resulted in significant increase in cell viability (84.5%), restored membrane integrity, reactive oxygen species level were maintained and cell cycle phases were normal. PC significantly up-regulated the activity of detoxification enzymes: heme oxygenase 1 (HO-1), glutathione transferase, glutathione peroxidase, superoxide dismutase and quinone reductase. Nrf2 translocation into the nucleus was also observed by immunocytochemistry analysis.

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Immunology/Inflammation

NOS

NO Synthase|NOS|Nrf2

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134-04-3

306.7

C15H11ClO5

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (326.05 mM)

[Cl-].Oc1ccc(cc1)-c1[o+]c2cc(O)cc(O)c2cc1O

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(-20℃)

With Ice Pack

≥ 2 years